Ebselen Protects against Oxidative and Morphological Effects of High Concentration Chronic Toluene Exposure on Rat Sciatic Nerves

نویسندگان

  • Ömer Coşkun
  • Mehmet Yüncü
  • Mehmet Kanter
  • Sadık Büyükbaş
چکیده

Aim: Neuroprotective effects of ebselen against free radical damage have been studied extensively. Toluene generates reactive oxygen species (ROS) and the toxic effects relating to these reactants. The aim of this study was designed to investigate the effects of chronic toluene inhalation in high concentration on lipid peroxidation, antioxidant enzyme activities and ultrastructural changes in the sciatic nerves of rats. Methods: We also examined the protective effects of ebselen against toluen exposure. Male Wistar albino rats (150-250 g) were divided in three experimental groups: the control, toluene and toluene+ebselen treated group (n=10 for each). Toluene treatment was performed by inhalation of 3000 ppm toluene, in a 8 hr/day and 6 day/week order for 16 weeks. Control group received 1ml serum physiologic and ebselen was given i.p. (10 mg/kg) to toluene+ebselen treated group just after toluene exposure per day. Blood and tissue samples were obtained for biochemical and histopathological investigation. The blood and sciatic nerves were assayed for toluene by gas chromatography. Results: Toluene significantly increased blood and tissue malondialdehyde (MDA), and decreased tissue superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), but not tissue catalase (CAT) levels when compared with controls. Ebselen administration prevented to increase of blood and tissue MDA and decrease to SOD and GSH-Px levels induced by toluene inhalation. Electron micrographs of sciatic nerve in the toluene group shows myelin destructions with onion-bulb and bubble form protrusion on the myelin sheath and axolemma border of myelinated axons. Furthermore, ebselen salvaged the nerve fibers from toluene exposure. Conclusion: Ebselen treatment may provide neuroprotection against toluene neurotoxicity by directly scavenging ROS and by indirectly augmenting their antioxidant capacity.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Additive pro-oxidative effects of methylmercury and ebselen in liver from suckling rat pups.

Oxidative stress has been pointed as an important molecular mechanism for liver injury in methylmercury (MeHg) poisoning. Ebselen, a seleno-organic compound that possesses anti-oxidant properties, is a useful therapeutic agent used in clinical situations involving oxidative stress. Here, we examined the possible in vivo protective effect of ebselen against the pro-oxidative effects of MeHg in l...

متن کامل

Troxerutin Chronic Treatment Protects against Fructose-induced Metabolic Syndrome in Male Rats

Background and Aims: Metabolic syndrome, a common metabolic disorder, for the serious health consequences such as insulin resistance and lipid abnormalities has been considered as a major clinical challenge with obscure causes. Oxidative stress is an important component of metabolic syndrome, contributing in its development. Troxerutin, a semi-synthetic derivative of natural bioflavonoid rutin,...

متن کامل

Vitamin E protects rat testis, eye and erythrocyte from oxidative stress during exposure to radiofrequency wave generated by a BTS antenna model

Background: Radio frequency wave (RFW) generated by mobile phones and wireless communication systems has been reported to cause adverse effects on reproductive function, vision and hematological parameters, possibly through oxidative stress. The aim of this study was to evaluate the effect of RFW generated bybase transceiver station BTS on oxidative stress in testis, eye and erythrocyte, and th...

متن کامل

P 15: Forced Exercise Attenuates Neuropathic Pain in Chronic Constriction Injury Male Rat: An Approach to Oxidative Stress and Inflammation

Introduction: Peripheral and central nerve injuries cause chronic neuropathic pain in many injured people besides motor disability. Exercise, as a behavioral and non-pharmacological treatment, has beneficial effects on people’s general health both for healthy and sick people. Therefore, this study was conducted to examine the effects of exercise on neuropathic pain induced by chronic cons...

متن کامل

P-16: Effect of Vitamin E and Green Tea Extracts on Semen Quality and Oxidative Stress Parameters in Rat Exposed to Cadmium

Background: Cadmium is a potential pollutant of the environment. It manifests cyto-toxic effects in different organs in animals. In the testis, changes due to disruption of the blood-testis barrier and oxidative stress have been noted. Therefore, the present study was carried out to investigate the potential protective effects of vitamin E and green tea extract in combination against cadmium (C...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2011